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The use of cell systems to evaluate whether the test product is cytotoxic or to evaluate the effect of the test product on cytotoxicity after metabolic enzyme metabolism (i.e. increased toxicity, attenuated toxicity, or no effect) does not provide a concentration reference for subsequent tests.
Incubation system | primary hepatocytes 3T3 cell 3T3 cells and liver microsomes |
Species | Human or animal |
Test concentration | 3-8, which is designed according to the customer's drug efficacy and toxicity data |
Cell density | Liver cells: 0.5-0.7×106 cells/mL 3T3 cells: 5-8×104 cells/mL Liver microsomes: 0.5-1 mg/mL |
Incubation time | Hepatocytes: 2-3 days (available on request) 3T3 cells: 4-24 hours (available on request) Liver microsomal: 30-120 minutes (according to the previous microsomal metabolic stability data) |
Analysis methods | Enzyme label instrument |
Data presentation | relative activity (% of VC) IC50 |