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In Vitro Study for Liver Cytotoxicity

In Vitro Study for Liver Cytotoxicity

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021-50800743

The use of cell systems to evaluate whether the test product is cytotoxic or to evaluate the effect of the test product on cytotoxicity after metabolic enzyme metabolism (i.e. increased toxicity, attenuated toxicity, or no effect) does not provide a concentration reference for subsequent tests.


Incubation system                 

primary hepatocytes

3T3 cell

3T3 cells and liver microsomes

Species

Human or animal

Test concentration

3-8, which is designed according to the customer's drug efficacy and toxicity data

Cell density

Liver cells: 0.5-0.7×106 cells/mL

3T3 cells: 5-8×104 cells/mL

Liver microsomes: 0.5-1 mg/mL

Incubation time

Hepatocytes: 2-3 days (available on request)

3T3 cells: 4-24 hours (available on request)

Liver microsomal: 30-120 minutes (according to the previous microsomal metabolic stability data)         

Analysis methods

Enzyme label instrument

Data presentation

relative activity (% of VC)

IC50


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