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Equilibrium dialysis/ultrafiltration was used to evaluate the binding rate of a test product to human and animal plasma/other histamins to accurately evaluate the free test concentration and provide parameters for the safety and efficacy of the drug.
Incubation system | Plasma |
Species | CD1 Mouse, SD Rat, Beagle, Crab-eating Monkey, Miniature Pig, New Zealand Rabbit, Human (other species or strains available upon request) |
Test concentration | 3 concentrations, which were designed according to the drug efficacy concentration |
Incubation system protein concentration | To Be Determined (available on request) |
Incubation time | Balance dialysis method: 4-6 hours (or available on request) Ultrafiltration: 10-30 minutes (or available on request) Overspeed centrifugation: incubation at 37℃ for 30 minutes 230000g centrifugation for 4-6 hours (or available according to different requirements) |
Analytical method | LC-MS/MS |
Data presentation | Equilibrium dialysis method: Protein binding rate recovery stability Ultrafiltration method: Nonspecific binding (NSB) Protein binding rate recovery Stability or other parameters Ultrafast centrifugal method: Protein binding rate |