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Through metabolite identification test, the main metabolites of the test substance in the incubation system are evaluated, the metabolic sites and metabolic pathways of the test substance are understood, and the reference is provided for the selection of subsequent toxicological animal species.
Incubation system | Subcellular components: microsomes, S9 or homogenate of liver, intestine, lung or kidney and othertissues Cell components: hepatocytes, human epithelial cell HACAT Gene recombinase system Other tissues, cells or subcellular components: can be provided according to requirements (such as plasma, etc.) In vivo samples: such as plasma, urine, feces or bile |
Species | CD1 mice, SD rats, beagle dogs, cynomolgus monkeys, minipigs, New Zealand rabbits, human (other species or strains can be provided according to requirements) |
Test concentration of the test substance | To Be Determined (different test concentrations can be provided according to requirements) |
Protein concentration of the incubation system | To Be Determined (different protein concentrations can be provided according to requirements) |
Incubation time | To Be Determined (can be provided according to different requirements) |
Coenzyme factors | Subcellular components or gene recombinase system: NADPH and/or UDPGA (other coenzyme factors can be provided according to requirements) |
Analysis method | Mass spectrometer detection |
Data presentation | Possible metabolite structure Mass spectrometry or ultraviolet relative abundance of metabolites Metabolic pathway Species difference or other parameters |